Glycerol
Glycerol can be measured in both Clinical Research and the Industrial, Commercial & Biotechnology applications.
Please see examples below.
Clinical & Research
Glycerol is normally measured in clinical research applications
Industry & Biotechnology
Glycerol can be measured in beverage analyses and monitored in fermentation and culture control applications.
Clinical & Research
A simple, rapid and direct enzymatic analysis for glycerol in plasma or serum.
Principle
In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide:
i)
ii)
Under the conditions of the assay, reactions i) and ii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to plasma glycerol concentration
Technical Summary
| Samples | Serum or plasma containing heparin, EDTA or oxalate anticoagulants |
| Sample Volume | 25 µl |
| Analysis Time | 20 - 25 seconds |
| Linearity | 0.2 - 1.0 mmol/L (0 - 10mg/dl) |
| Precision | Within run: 0.25 mmol/L (ca. 2.3 mg/dl) |
| Detection Limit | 0.1 mmol/L (ca. 0.9 mg/dl) |
| Reagent Stability | Shelf-life unopened: 12 months stored at 0 - 5°C. Shelf-life reconstituted: 3 - 4 weeks stored at 0 - 5°C. |
Notes:
Levels below ca 0.2 mmol/L (1.8 mg/dl) can be regarded as clinically normal for human plasma.
This kit includes a liquid QC.
Analysers
Technical Bulletins
Industry & Biotechnology
A simple, rapid and direct enzymatic analysis for glycerol in a wide variety of aqueous fluids including beverage, pharmaceutical and fermentation extracts.
Principle
In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide:
i)
ii)
Under the conditions of the assay, reactions i) and ii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to plasma glycerol concentration.
Technical Summary
| Samples | Beverages, pharmaceuticals, fermentation extracts |
| Sample Volume | 7 µl (variable 2 - 11 µl) |
| Analysis Time | 20 - 25 seconds |
| Analytical Range | 0.1 – 4.0% W/V (1.0 – 40.0 g/L) |
| Precision | S.D. = 0.01 at 2 %W/V |
| Reagent Stability | Shelf-life greater than 12 months unopened when stored at 0 - 5°C, 3- 4 weeks after reconstitution if stored at 0 - 5°C between use. |
Notes:
The Kit insert contains instructions on how to use this assay for low level (<0.1% W/V) concentrations of Glycerol.
