Glucose
Glucose can be measured in both Clinical Research and Industrial, Commercial & Biotechnology applications
Clinical & Research
Glucose can be measured in these areas:
- Diabetes monitoring
- Diabetes clamp studies
- Clinical pathology
Industry & Biotechnology
Glucose can be measured in these areas:
- Biotechnology and pharmaceutical analysis
- Food and beverage analysis
Clinical & Research
Analox offer two appraches to clinical glucose measurement. The first is a very fast enzymatic assay for glucose in plasma, serum or whole blood via Analox collection systems.
The second using the GM9D (Glucose Direct) Analyser eliminates the need for collection systems, as all the additives have been added to the reagent buffer so that unmodified whole blood can be injected directly.
Principle
In the presence of molecular oxygen, ß-D-glucose is oxidised by the enzyme glucose oxidase (GOD) to gluconic acid and hydrogen peroxide,
Under the condtions of the assay, the rate of oxygen consumption is directly proportional to glucose concentration.
Technical Summary
| Samples | Plasma, serum, whole-blood collected in Analox tubes or capillaries, C.S.F., urine (suitably diluted) and other aqueous media. |
| Sample Volume | 10 µl (variable 3.5 - 25 µl) |
| Analysis Time | 20 seconds |
| Linearity | 0- 30 mmol/L (10 µl sample); 0 - 540 mg/dl (10 µl sample) 0- 50 mmol/L (5 µl sample); 0- 900 mg/dl (5 µl sample) |
| Precision | Within run: C.V. of 1.0% @ 5 mmol/L (Plasma) C.V. of 1.4% @ 10 mmol/L (Plasma) C.V. of 0.85% @ 12 mmol/L (Whole Blood) |
| Reagent Stability | Shelf-life unopened: 18 months stored at 0 - 5°C |
Notes:
Glucose standards at 2.5, 5.0, 8.0 and 25.0 mmol/L (30 ml) are available and ordered as required.
Other values at 30.0 and 50.0 mmol/L can be supplied to special order.
Industry & Biotechnology
A very fast enzymatic assay for ß-D-glucose (dextrose) in beverages, biotechnology and biopharmaceutical applications
Principle
In the presence of molecular oxygen, ß-D-glucose is oxidised by the enzyme glucose oxidase (GOD) to gluconic acid and hydrogen peroxide,
Under the condtions of the assay, the rate of oxygen consumption is directly proportional to glucose concentration.
Technical Summary
| Samples | Any suitable homogenous biological fluid or aqueous extract. Gross particulates should be removed by course filtration or centrifugation. However, turbidity or opacity does not cause interference |
| Sample Volume | 10 µl (25 µl for GM10) |
| Analysis Time | 20 seconds |
| Analytical Range | 0.05 - 20 %W/V (for GM8, GL6); 0.20 - 40 %W/V (for GM10) |
| Analytical Cycle | Approx. 60 seconds |
| Precision | C.V. of 1.0% at ca. 0.1% W/V |
| Reagent Stability | Shelf-life unopened: 18 months stored at 0 - 5°C. |
Notes:
Long-life glucose standards (with preservative) at 1.0, 2.0, 5.0, 8.0 and 20.0 %W/V (30 ml) are available and ordered as required. Other values can be supplied to special order.
Analysers
No analysers are currently available for this analyte.
