Ethanol
Ethanol can be measured in both Clinical Research and in the Industrial, Commercial & biotechnology applications.
Please see below.
Clinical & Research
Ethanol can be measured in these areas:
- Clinical Pathology
- Alcoholism Research
Industry & Biotechnology
Ethanol can be measured in these areas:
- Biotechnology & pharmaceutical applications
- Food & beverage applications
- Toiletry manufacture
Clinical & Research
A rapid high performance plasma alcohol (ethanol) analysis using Alcohol Oxidase (AOD).
Principle
In the presence of molecular oxygen, ethanol is oxidised by the enzyme alcohol oxidase to acetaldehyde and hydrogen peroxide:
Technical Summary
| Samples | Plasma, serum, urine, precipitated whole blood (neutralised) |
| Sample Volume | 5 µl (variable 2.5 - 25 µl) |
| Analysis Time | 20 seconds |
| Linearity | 200 mg/dl (43 mmol/L) for 5 µl samples 400 mg/dl (86 mmol/L) for 2.5 µl samples |
| Precision | Typical within-run C.V. = 2.5 % @ 85 mg/dl (whole blood) |
| Reagent Stability | Shelf-life 6 months unopened when stored at 0 - 5°C. Reconstituted AOD/buffer reagent, ca 5 days if stored at 0 - 5°C between use. |
Notes:
Alcohol oxidase is non-specific towards ethanol. This assay will also detect other low molecular weight alcohols, e.g. methanol.
Analysers
Technical Bulletins
Industry & Biotechnology
A rapid high performance ethanol analysis based on the direct reaction with the enzyme Alcohol Oxidase (AOD). Applications include brewing, flavourings, pharmaceuticals, toiletries etc.
Principle
In the presence of molecular oxygen, ethanol is oxidised by the enzyme alcohol oxidase to acetaldehyde and hydrogen peroxide
Under the conditions of the assay, oxygen consumption is directly proportional to ethanol concentration.
Technical Summary
| Samples | Aqueous samples including beers, wines, cough mixtures, tonics, shampoos |
| Sample Volume | 5µl |
| Analysis Time | 20 - 25 seconds |
| Analytical Range | 0.1 - 10 %VV (AM3); 0.2 - 40 %VV (AM2) |
| Precision | Typical within-run S.D. = 0.055 @ 9 %VV |
| Reagent Stability | Shelf-life unopened: 6 months stored at 0-5°C. Shelf-life reconstituted: AOD/buffer reagent, ca. 5 days stored at 0-5°C. |
Notes:
i) Alcohol oxidase is non-specific towards ethanol. This assay will also detect other low molecular weight alcohols, e.g. methanol.
ii) Primary calibrating standards, 0.1 - 40 %VV are available separately, as required.
Analysers
No analysers are currently available for this analyte.
